Figure 7.

The influence of Az on immune system. The effect of 7-day intramuscular Az (0.15 and 0.5 mg/kg) administration to male ICR mice (a) on their delayed-type hypersensitivity (DTH) to a specific antigen (10 mM trinitrobenzenesulfonic acid) manifested by paw edema, (b) on their immune response to a bovine serum albumin (BSA) and (c) on phagocytic activity of their peritoneal macrophages. (a) The presented indexes of DTH reactions reflect the normalized difference in the weights of treated and control mouse hind paws. (b) The titers of IgG in blood serum of mice from experimental and control groups after their standard immunization with BSA are presented. (c) The number of total and phagocytic (engulfing ink particles) macrophages in 1 μL of peritoneal exudate isolated from experimental and control animals. In all three tests, there was no significant difference between control and experimental animals (p > 0.05, Kruskall-Wallis ANOVA on ranks) in the parameters studied. The results are presented as mean values ± SEM, n = 5–10.