Table A1.
Summary of freshwater sample collection dates, locations, water body types, identified taxa, scum presence, enumerated cells in comparison to 20,000 cells/mL threshold and total microcystin concentrations (extra and intracellular combined) during 2016 throughout England.
| Sample | Date Collected | Grid Reference | Size (km2) | Type | Taxa | Scum | Threshold Exceeded | Total MCs (μg/L) | Total MCs (pg/Cell ‡) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 09/02/16 | SD4648304966 | 0.0024 | NL | M | N | N | 0.0 | 304 |
| 2 | 31/03/16 | SJ7500781064 | 0.08 | NL | Ap Os | Y | y | 22.7 | 45 |
| 3 | 20/04/16 | SK4764995980 | 0.18 | R | An | Y | y | 610.5 | 1221 |
| 4 | 05/05/16 | SU0415037183 | 0.03 | NL | Os | Y | y | 7.1 | 14 |
| 5 | 09/05/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 6 | 09/05/16 | TQ1730003400 | 0.009 | NL | Os | N | n | 0.0 | 0.0 |
| P-1 | 12/05/16 | ST4637977160 | 0.006 | AL | An | N | Y | 4019.4 | na |
| P-2 | 12/05/16 | ST4637977160 | 0.006 | AL | An | Y | Y | 0.8 | na |
| P-3 | 12/05/16 | ST4637977160 | 0.006 | AL | M | N | N | 0.8 | na |
| P-4 | 12/05/16 | ST4637977160 | 0.006 | AL | M | Y | Y | 0.0 | na |
| 7 | 17/05/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 9 | 17/05/16 | TQ5453672982 | 0.005 | NL | Os | N | n | 6.7 | 67,182 |
| 8 | 18/05/16 | TQ5453672982 | 0.005 | NL | Os | N | n | 0.0 | 0.0 |
| 10 | 23/05/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.7 | na |
| 11 | 23/05/16 | SE3999828451 | 0.06 | NL | Pl | n | y | 0.0 | 0.0 |
| 12 | 23/05/16 | SE4710227289 | 0.09 | NL | Os | n | y | 0.0 | 0.0 |
| 13 | 23/05/16 | SE4535127829 | 0.18 | NL | Os | n | y | 0.0 | 0.0 |
| 14 | 03/06/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.1 | na |
| 15 | 08/06/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 16 | 08/06/16 | SK6175739286 | 0.4 | AL | Os An | N | y | 0.04 | 0.3 |
| P-5 | 08/06/16 | ST4637977160 | 0.006 | AL | M | N | N | 8.0 | 668 |
| P-6 | 08/06/16 | ST4637977160 | 0.006 | AL | M | Y | Y | 9.0 | 751 |
| 17 | 10/06/16 | TR0318143471 | 0.04 | NL | An | N | y | 0.0 | 0.0 |
| 18 | 12/06/16 | SE2221504615 | 0.09 | R | Pl | Y | y | 0.2 | 0.4 |
| 19 | 12/06/16 | SE2204504797 | 0.09 | R | Pl | Y | y | 0.1 | 0.3 |
| 21 | 13/06/16 | SD3754089840 | 5 | NL | An Os | Y | y | 1.5 | 3.0 |
| 20 | 14/06/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.8 | na |
| 22 | 17/06/16 | TR0318143471 | 0.04 | NL | An | N | y | 0.0 | 1.7 |
| 23 | 21/06/16 | TQ4769380228 | 0.03 | AL | Ap Go | Y | y | 1.0 | 2.0 |
| 26 * | 23/06/16 | TQ8153583675 | 0.0012 | AL | An | Y | y | 0.0 | 0.0 |
| 29 | 24/06/16 | SU5071867008 | 0.0225 | NL | Ap | Y | y | 0.0 | 0.0 |
| 24 | 26/06/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 30 | 26/06/16 | SU8478040480 | 0.16 | NL | M Gl | Y | y | 0.4 | 0.8 |
| 25 | 28/06/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 27 | 28/06/16 | TQ4769380228 | 0.03 | AL | Ap | Y | y | 0.5 | 1.0 |
| 28 | 28/06/16 | TQ0390677601 | 0.01 | NL | An | Y | y | 0.6 | 1.2 |
| 31 | 30/06/16 | ST2975437518 | 0.0075 | AL | Os | Y | y | 1.9 | 3.9 |
| 32 | 30/06/16 | TR2268161365 | 0.004 | AL | Os An | Y | y | 0.1 | 0.2 |
| 33 | 04/07/16 | TQ4769380228 | 0.03 | AL | Ap | Y | y | 0.0 | 0.0 |
| 34 | 04/07/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 35 | 06/07/16 | SK5215608209 | 0.09 | NL | An | Y | y | 6.7 | 13 |
| 43 † | 06/07/16 | TQ9163916682 | 0.0003 | NL | Gy | N | n | 0.0 | 0.0 |
| 36 | 07/07/16 | TR2268161365 | 0.004 | AL | Os An | N | n | 0.1 | 92 |
| 37 * | 08/07/16 | TL1775658154 | 0.03 | NL | An | Y | y | 0.0 | 0.3 |
| 38 | 11/07/16 | SU8478040480 | 0.16 | NL | M Gl | Y | y | 1.2 | 2.4 |
| 41 | 12/07/16 | SJ3791791961 | 0.0075 | NL | An | N | n | 0.9 | 18133 |
| 39 | 13/07/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 40 | 14/07/16 | TR2268161365 | 0.004 | AL | An Os | Y | y | 1.8 | 13 |
| 42 | 14/07/16 | TQ4769380228 | 0.03 | AL | Ap | Y | y | 0.0 | 0.0 |
| 44 | 18/07/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 45 | 18/07/16 | SD4881461153 | 0.0003 | NL | An | Y | y | 0.0 | 0.0 |
| 46 | 21/07/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 47 | 24/07/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.7 | na |
| 48 | 25/07/16 | SJ6844577250 | 0.125 | NL | M An | n | n | 0.1 | 10 |
| 52 | 25/07/16 | SU0686094628 | 0.24 | NL | Gl | y | y | 0.0 | 0.0 |
| 53 | 25/07/16 | SP5732550449 | 0.006 | NL | Ap | y | y | 0.0 | 0.0 |
| 50 | 26/07/16 | TA0848030328 | 0.0005 | AL | M | y | y | 90.9 | 182 |
| 54 | 26/07/16 | SD4881461153 | 0.0003 | NL | An | y | y | 0.0 | 0.0 |
| 55 * | 26/07/16 | TR0808521709 | 0.0008 | AL | M An | n | y | 0.5 | 0.1 |
| 49 | 27/07/16 | SP8896035736 | 0.09 | NL | Ap | y | y | 0.1 | 0.3 |
| 51 | 27/07/16 | SU8478040480 | 0.16 | NL | Gl | y | y | 0.0 | 0.0 |
| 56 | 27/07/16 | SJ2926290282 | 0.0075 | AL | M | n | n | 2.8 | 234 |
| 57 | 28/07/16 | SU0927095489 | 0.0024 | NL | An | n | y | 0.0 | 0.0 |
| 58 | 01/08/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 59 | 01/08/16 | SP9990079600 | 0.4 | NL | M | Y | y | 193.6 | 387 |
| 60 | 01/08/16 | SE5809049247 | 0.0225 | NL | An | Y | y | 0.0 | 0.0 |
| 61 | 02/08/16 | TR0808521709 | 0.0008 | AL | M | N | y | 0.6 | 0.2 |
| 62 | 04/08/16 | TA1094628599 | 0.0036 | AL | An Os | N | y | 3.3 | 62 |
| 63 | 04/08/16 | SD7415406707 | 0.0225 | AL | Ap M | Y | y | 30.8 | 62 |
| 64 | 05/08/16 | SD4017798465 | 5 | An | Y | y | 0.2 | 0.3 | |
| 65 | 09/08/16 | TR0808521709 | 0.0008 | AL | M | N | n | 1.2 | 167 |
| 67 | 09/08/16 | TR0318143471 | 0.04 | NL | Ap | Y | y | 0.1 | 0.2 |
| 66 | 11/08/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 68 | 12/08/16 | SK5282738907 | 0.06 | NL | An M | Y | y | 7.2 | 14 |
| 81 | 15/08/16 | SO8307960032 | 0.00125 | AL | An Os | N | y | 0.0 | 0.0 |
| 82 | 15/08/16 | SO8315359973 | 0.025 | AL | An Os | N | y | 0.0 | 0.0 |
| 80 | 17/08/16 | SO7499006989 | 0.12 | NL | Ap M An At | Y | y | 7.9 | 15.8 |
| 78 | 18/08/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 85 | 18/08/16 | SD9089540319 | 0.004 | AL | M Go | N | y | 1.1 | 2.2 |
| 79 | 19/08/16 | TG2522007900 | 0.2 | NL | Ap M An | N | n | 0.1 | 1046 |
| 83 | 22/08/16 | TR0318143471 | 0.04 | NL | Ap | Y | y | 0.8 | 1.6 |
| 86 | 22/08/16 | ST4679744377 | 0.005 | AL | An M Os | Y | y | 2.3 | 4.5 |
| 84 | 23/08/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 88 | 23/08/16 | SZ3777596540 | 0.035 | NL | M | Y | y | 2.8 | 5.6 |
| 87 | 25/08/16 | SD9089540319 | 0.004 | AL | M Go | Y | y | 10.0 | 20 |
| 89 | 25/08/16 | SU8478040480 | 0.16 | NL | Gl | Y | y | 0.0 | 0.0 |
| 90 | 25/08/16 | SU8201355304 | 0.09 | NL | Ap | Y | y | 0.0 | 0.0 |
| 99 | 25/08/16 | SU8728403186 | 0.16 | NL | M Ap | Y | y | 78.7 | 157 |
| 101 | 25/08/16 | SU8728403186 | 0.16 | NL | M | Y | y | 296.9 | 594 |
| 102 | 25/08/16 | SU8728403186 | 0.16 | NL | M Ap | N | y | 1.5 | 3.0 |
| 100 | 26/08/16 | SU8728403186 | 0.16 | NL | Ap | Y | y | 0.1 | 0.2 |
| 91 | 27/08/16 | TL1572653882 | 0.01 | NL | M | Y | y | 13.6 | 27 |
| 92 | 30/08/16 | TR0318143471 | 0.04 | NL | Ap M An | Y | y | 2561.0 | 5122 |
| 93 | 30/08/16 | TL6943690325 | 0.0009 | AL | M | Y | y | 2.5 | 5.0 |
| 97 | 30/08/16 | SU6663501299 | 0.0036 | NL | Ap Ps | N | y | 3.2 | 21 |
| 94 | 01/09/16 | SD9089540319 | 0.004 | AL | M Go | Y | y | 89.9 | 180 |
| 95 | 02/09/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | na |
| 98 | 02/09/16 | SU4410721883 | 0.004 | NL | M An | Y | y | 21.1 | 42 |
| 96 | 04/09/16 | TQ2097086918 | 0.16 | R | Ap | Y | y | 2.2 | 4.4 |
| 107 | 06/09/16 | SU1762682163 | 0.175 | NL | M | Y | y | 14.2 | 28 |
| 108 | 06/09/16 | SU1762682163 | 0.175 | NL | Ap | N | n | 0.0 | 0.0 |
| 103 | 07/09/16 | SO7499006989 | 0.12 | NL | M Ap | Y | y | 103.2 | 206 |
| 104 | 07/09/16 | TQ3403669313 | 0.0096 | NL | Ap | Y | y | 0.0 | 0.0 |
| 106 | 07/09/16 | SU5296391042 | 0.0024 | NL | At | N | y | 0.0 | 0.1 |
| 105 | 09/09/16 | SE3248717960 | 0.3 | NL | M Ap | Y | y | 0.3 | 0.6 |
| 111 | 12/09/16 | SU8779803166 | 0.015 | NL | M An | Y | y | 244.0 | 488 |
| 114 | 12/09/16 | SD9089540319 | 0.004 | AL | M Go | Y | y | 42,724.1 | 85,448 |
| 110 | 13/09/16 | SO8267761829 | 0.01 | AL | Ap An M | Y | y | 52.7 | 105 |
| 112 | 13/09/16 | TL538202 | 0.0075 | NL | An | Y | y | 0.2 | 0.5 |
| 115 | 13/09/16 | SU7483471048 | 0.015 | NL | An Ps Os | N | y | 0.0 | 0.0 |
| 118 | 13/09/16 | SU1762682163 | 0.175 | NL | Ap | Y | y | 0.2 | 0.3 |
| 109 | 14/09/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.2 | na |
| 119 | 14/09/16 | SJ7493757095 | 0.01 | NL | An M | Y | y | 830.4 | 1661 |
| 122 | 14/09/16 | TL 23456 12000 | 0.01 | NL | M | N | n | 0.3 | 2706 |
| 116 | 14/09/16 | SU0158594707 | 0.045 | AL | M An | Y | y | 1.8 | 3.7 |
| 113 | 15/09/16 | SD2430078100 | 0.18 | R | Go M An | Y | y | 1.0 | 2.0 |
| 117 | 15/09/16 | SP4976352470 | 0.12 | R | Ap M An Os | Y | y | 1.9 | 3.9 |
| 120 | 16/09/16 | TQ3403669313 | 0.0096 | NL | Ap | Y | y | 1.1 | 2.2 |
| 123 | 19/09/16 | TL 23456 12000 | 0.01 | NL | M | N | n | 1.0 | 9926 |
| 121 | 20/09/16 | TG2522007900 | 0.2 | NL | Sn Os M Me | N | n | 0.0 | 0.0 |
| 125 | 21/09/16 | TQ6421836460 | 0.015 | NL | Os Go | Y | y | 7.1 | 14.2 |
| 124 | 22/09/16 | ST5805477480 | 0.009 | NL | - | N | n | 0.0 | 238 |
| 126 | 22/09/16 | TQ3403669313 | 0.0096 | NL | Ap | Y | y | 0.0 | 0.1 |
| 128 | 29/09/16 | SD9089540319 | 0.004 | AL | Go M | Y | y | 2.3 | 4.6 |
| 132 | 07/10/16 | TL0072233302 | 0.0015 | NL | An | Y | y | 0.3 | 5.7 |
| 133 | 10/10/16 | SD9089540319 | 0.004 | AL | Go M | Y | y | 4.1 | 8.3 |
| 129 | 11/10/16 | SO8745754176 | 0.0008 | AL | Os M | Y | y | 0.0 | 0.0 |
| 130 | 11/10/16 | SK4542133791 | 0.025 | NL | Pl | N | y | 0.0 | 0.0 |
| 131 | 11/10/16 | SK4542133791 | 0.025 | NL | Pl | N | y | 0.0 | 0.0 |
| 135 | 13/10/16 | TQ8074010542 | 0.002 | NL | Go Os Ly | N | y | 0.4 | 1.2 |
| 136 | 13/10/16 | TQ8032210477 | 0.004 | R | Go | N | y | 0.7 | 0.8 |
| 134 | 20/10/16 | ST8529642290 | 0.04 | NL | Go Ap Os | Y | y | 278.8 | 558 |
| 137 | 24/10/16 | SE0209118762 | 0.12 | R | Ap | Y | y | 5.5 | 11.0 |
| 138 | 26/10/16 | SD9089540319 | 0.004 | AL | M Ap | N | n | 0.5 | 309 |
| 139 | 31/10/16 | SD7146329903 | 0.18 | R | Go | Y | y | 198.8 | 398 |
| 140 | 07/12/16 | SX1799070900 | 0.001 | NL | M Os | Y | y | 7.2 | 14 |
| 141 | 13/12/16 | NY3750003300 | 3.2 | NL | Go | Y | y | 0.0 | 0.0 |
* Samples taken from water bodies associated with fish kills, nd = not detected, MCs = microcystins; † Brackish water, ‡ Assuming scum containing samples have 500,000 cells/mL; NL = Natural lake, AL = artificial lake, R = reservoir, na = no analysis. Cyanobacterial genus abbreviations as follows: M = Microcystis, An = Anabaena, Ap = Aphanizomenon, Os = Oscillatoria, Pl = Planktothrix, Go = Gomphosphaeria, Gl = Gloeotrichia, Gy = Gymnodinium, Ly = Lyngbia, At = Aphanothece, Ps = Pseudoanabaena, Sn = Snowella, Me = Merismopedia.