Table 5.
Bio-Plex cytokine information for detection of pig proinflammatory cytokines in sera.
| Cytokine | Capture Antibody; Supplier | Detection Antibody; Supplier; Dilution | Standard; Supplier; Initial Concentration | Bead; Supplier |
|---|---|---|---|---|
| IL1β | MAb anti porc IL1β/IF2; R & D MAB6811 |
Goat anti porc IL1β/IF2 biotin; R & D BAF681; 0.5 μg/mL |
recombinant porc IL1β/IF2; R & D 681-PI-10; 5000 pg/mL |
Region 26; BioRad MC10026-01 |
| IL8 | MAb anti sheep IL8 (86.9% homology); AbD Serotec MCA1660 |
MAb anti porc CXCL8/IL8; R & D MAB5351; biotinylated in house; 1/400 dilution |
Recombinant porc IL-8; Kingfisher RP0109S-005; 200 pg/mL | Region 27; BioRad MC10027-01 |
| IL13 | Goat anti swine IL-13; Kingfisher PB0094S-100 | Goat anti swine IL-13 biotin; Kingfisher PBB0096S-050; 0.5 μg/mL |
Recombinant swine IL-13; Kingfisher RP0007S-005; 5000 pg/mL |
Region 52 ; BioRad MC10052-01 |
| TNFα | MAb anti porcine TNFα; R&D MAB6902 |
Goat anti porcine TNF α biotin; R & D BAF690; 0.5 μg/mL |
Recombinant porcine TNFα; R & D 690-PT-025; 5000 pg/mL |
Region 34; BioRad MC10034-01 |
| IFNγ | MAb anti-porcine IFNγ; Fisher ENMP700 |
MAb anti-porc IFNγ; Fisher ENPP700; biotinylated in-house; 1/400 dilution |
Recombinant porcine IFNγ; Ceiba Geigy (gift); 2000 pg/mL |
Region 43; BioRad MC10043-01 |