Table 3.
Summary of PANTHER Overrepresentation Test of the 796 unique differentially expressed (DE) genes in livers of Eastern wild turkeys compared to controls after AFB1 exposure.
| Category | Gallus gallus—REFLIST Genes (15,789) | Observed Turkey Genes | Expected | over/under | Fold Enrichment | p-Value |
|---|---|---|---|---|---|---|
| GO biological process complete | ||||||
| negative regulation of neurogenesis (GO:0050768) | 101 | 12 | 2.15 | + | 5.58 | 1.28 × 10−2 |
| negative regulation of cell development (GO:0010721) | 119 | 13 | 2.53 | + | 5.13 | 1.21 × 10−2 |
| negative regulation of nervous system development (GO:0051961) | 111 | 12 | 2.36 | + | 5.08 | 3.29 × 10−2 |
| regulation of system process (GO:0044057) | 184 | 16 | 3.92 | + | 4.09 | 1.58 × 10−2 |
| regulation of membrane potential (GO:0042391) | 185 | 16 | 3.94 | + | 4.06 | 1.69 × 10−2 |
| regulation of neuron differentiation (GO:0045664) | 233 | 19 | 4.96 | + | 3.83 | 4.83 × 10−3 |
| regulation of neurogenesis (GO:0050767) | 279 | 20 | 5.94 | + | 3.37 | 1.72 × 10−2 |
| regulation of nervous system development (GO:0051960) | 323 | 23 | 6.87 | + | 3.35 | 3.53 × 10−3 |
| neurological system process (GO:0050877) | 337 | 22 | 7.17 | + | 3.07 | 2.45 × 10−2 |
| system process (GO:0003008) | 559 | 30 | 11.9 | + | 2.52 | 2.36 × 10−2 |
| generation of neurons (GO:0048699) | 589 | 31 | 12.53 | + | 2.47 | 2.40 × 10−2 |
| neurogenesis (GO:0022008) | 636 | 33 | 13.53 | + | 2.44 | 1.57 × 10−2 |
| nervous system development (GO:0007399) | 886 | 44 | 18.85 | + | 2.33 | 1.03 × 10−3 |
| regulation of multicellular organismal process (GO:0051239) | 1083 | 52 | 23.05 | + | 2.26 | 2.01 × 10−4 |
| system development (GO:0048731) | 1613 | 64 | 34.33 | + | 1.86 | 4.44 × 10−3 |
| cell communication (GO:0007154) | 2250 | 85 | 47.88 | + | 1.78 | 3.50 × 10−4 |
| single organism signaling (GO:0044700) | 2201 | 83 | 46.84 | + | 1.77 | 5.71 × 10−4 |
| multicellular organismal process (GO:0032501) | 2417 | 91 | 51.44 | + | 1.77 | 1.16 × 10−4 |
| signaling (GO:0023052) | 2205 | 83 | 46.92 | + | 1.77 | 6.18 × 10−4 |
| multicellular organism development (GO:0007275) | 1814 | 68 | 38.6 | + | 1.76 | 1.37 × 10−2 |
| single-multicellular organism process (GO:0044707) | 2120 | 79 | 45.11 | + | 1.75 | 2.09 × 10−3 |
| anatomical structure development (GO:0048856) | 1971 | 71 | 41.94 | + | 1.69 | 3.16 × 10−2 |
| single-organism process (GO:0044699) | 6098 | 180 | 129.77 | + | 1.39 | 1.05 × 10−4 |
| Unclassified (UNCLASSIFIED) | 6357 | 84 | 135.28 | - | 0.62 | 0.00 × 10 |
| GO cellular component complete | ||||||
| sarcolemma (GO:0042383) | 43 | 7 | 0.92 | + | 7.65 | 4.23 × 10−2 |
| proteinaceous extracellular matrix (GO:0005578) | 168 | 14 | 3.58 | + | 3.92 | 1.85 × 10−2 |
| extracellular matrix (GO:0031012) | 249 | 18 | 5.3 | + | 3.4 | 8.51 × 10−3 |
| plasma membrane part (GO:0044459) | 987 | 45 | 21.0 | + | 2.14 | 1.33 × 10−3 |
| intrinsic component of membrane (GO:0031224) | 3244 | 132 | 69.03 | + | 1.91 | 3.19 × 10−12 |
| integral component of membrane (GO:0016021) | 3200 | 129 | 68.1 | + | 1.89 | 1.70 × 10−11 |
| plasma membrane (GO:0005886) | 1834 | 71 | 39.03 | + | 1.82 | 4.27 × 10−4 |
| membrane part (GO:0044425) | 3689 | 142 | 78.5 | + | 1.81 | 1.39 × 10−11 |
| cell periphery (GO:0071944) | 1888 | 72 | 40.18 | + | 1.79 | 6.08 × 10−4 |
| extracellular region part (GO:0044421) | 1555 | 57 | 33.09 | + | 1.72 | 3.49 × 10−2 |
| membrane (GO:0016020) | 4745 | 154 | 100.98 | + | 1.53 | 7.69 × 10−7 |
| organelle part (GO:0044422) | 3580 | 45 | 76.18 | - | 0.59 | 1.11 × 10−2 |
| intracellular organelle part (GO:0044446) | 3472 | 41 | 73.89 | - | 0.55 | 2.59 × 10−3 |
| nucleus (GO:0005634) | 3106 | 35 | 66.1 | - | 0.53 | 3.12 × 10−3 |
| organelle lumen (GO:0043233) | 1703 | 15 | 36.24 | - | 0.41 | 2.45 × 10−2 |
| intracellular organelle lumen (GO:0070013) | 1703 | 15 | 36.24 | - | 0.41 | 2.45 × 10−2 |
| membrane-enclosed lumen (GO:0031974) | 1703 | 15 | 36.24 | - | 0.41 | 2.45 × 10−2 |
| nuclear part (GO:0044428) | 1764 | 15 | 37.54 | - | 0.4 | 1.01 × 10−2 |
| Unclassified (UNCLASSIFIED) | 6062 | 78 | 129.0 | - | 0.6 | 0.00 × 10 |
| GO molecular function complete | ||||||
| transporter activity | 861 | 39 | 18.32 | + | 2.13 | 1.71 × 10−2 |
| nucleic acid binding | 1988 | 15 | 42.31 | - | 0.35 | 6.66 × 10−4 |
| Unclassified | 6653 | 112 | 141.58 | - | 0.79 | 0.00 × 10 |
Included categories had fold enrichment (number of DE genes divided by expected (Exp)) > 2.0. For each Gene Ontology category, the number of genes in the reference list and those differentially expressed in the turkey are given. DE turkey genes were matched to the chicken gene reference list for analysis in PANTHER [38]. p-values are as determined by the binomial statistic.