Table 4.
Summary of PANTHER Overrepresentation Test of the 1241 unique differentially expressed (DE) genes in liver of domesticated turkeys after AFB1 exposure as compared to controls.
| Category | Gallus gallus—REFLIST Genes (15789) | Observed Turkey Genes | Expected | over/under | Fold Enrichment | p-Value |
|---|---|---|---|---|---|---|
| GO biological process complete | ||||||
| organelle fission (GO:0048285) | 135 | 17 | 4.52 | + | 3.76 | 2.53 × 10−2 |
| oxidation-reduction process (GO:0055114) | 549 | 46 | 18.39 | + | 2.50 | 1.11 × 10−4 |
| regulation of immune system process (GO:0002682) | 408 | 33 | 13.67 | + | 2.41 | 2.40 × 10−2 |
| signal transduction (GO:0007165) | 2057 | 114 | 68.92 | + | 1.65 | 2.19 × 10−4 |
| Signaling (GO:0023052) | 2205 | 122 | 73.88 | + | 1.65 | 6.80 × 10−5 |
| single organism signaling (GO:0044700) | 2201 | 121 | 73.74 | + | 1.64 | 1.15 × 10−4 |
| single-organism metabolic process (GO:0044710) | 1745 | 95 | 58.47 | + | 1.62 | 8.40 × 10−3 |
| cell communication (GO:0007154) | 2250 | 122 | 75.38 | + | 1.62 | 2.13 × 10−4 |
| cellular response to stimulus (GO:0051716) | 2657 | 135 | 89.02 | + | 1.52 | 1.45 × 10−3 |
| single-organism process (GO:0044699) | 6098 | 309 | 204.31 | + | 1.51 | 1.54 × 10−16 |
| response to stimulus (GO:0050896) | 3224 | 159 | 108.02 | + | 1.47 | 5.26 × 10−4 |
| single-organism cellular process (GO:0044763) | 4263 | 205 | 142.83 | + | 1.44 | 1.46 × 10−5 |
| regulation of cellular process (GO:0050794) | 4810 | 220 | 161.16 | + | 1.37 | 2.08 × 10−4 |
| regulation of biological process GO:0050789) | 5131 | 227 | 171.91 | + | 1.32 | 1.83 × 10−3 |
| biological regulation (GO:0065007) | 5521 | 244 | 184.98 | + | 1.32 | 4.20 × 10−4 |
| cellular process (GO:0009987) | 7267 | 307 | 243.48 | + | 1.26 | 1.04 × 10−4 |
| Unclassified | 6357 | 130 | 212.99 | - | 0.61 | 0.00 × 10 |
| GO cellular component complete | ||||||
| mitochondrial inner membrane (GO:0005743) | 213 | 22 | 7.14 | + | 3.08 | 4.57 × 10−3 |
| mitochondrial membrane (GO:0031966) | 273 | 26 | 9.15 | + | 2.84 | 2.75 × 10−3 |
| mitochondrial envelope (GO:0005740) | 290 | 27 | 9.72 | + | 2.78 | 2.69 × 10−3 |
| organelle inner membrane (GO:0019866) | 239 | 22 | 8.01 | + | 2.75 | 2.60 × 10−2 |
| mitochondrial part (GO:0044429) | 395 | 30 | 13.23 | + | 2.27 | 3.65 × 10−2 |
| mitochondrion (GO:0005739) | 819 | 50 | 27.44 | + | 1.82 | 3.85 × 10−2 |
| membrane part (GO:0044425) | 3689 | 193 | 123.6 | + | 1.56 | 7.73 × 10−9 |
| cell periphery (GO:0071944) | 1888 | 98 | 63.26 | + | 1.55 | 7.45 × 10−3 |
| plasma membrane (GO:0005886) | 1834 | 95 | 61.45 | + | 1.55 | 1.15 × 10−2 |
| intrinsic component of membrane (GO:0031224) | 3244 | 166 | 108.69 | + | 1.53 | 2.94 × 10−6 |
| integral component of membrane (GO:0016021) | 3200 | 163 | 107.21 | + | 1.52 | 6.18 × 10−6 |
| Membrane(GO:0016020) | 4745 | 238 | 158.98 | + | 1.50 | 3.01 × 10−10 |
| Cell (GO:0005623) | 7948 | 328 | 266.29 | + | 1.23 | 4.09 × 10−5 |
| cell part (GO:0044464) | 7895 | 325 | 264.52 | + | 1.23 | 7.49 × 10−5 |
| Intracellular (GO:0005622) | 6948 | 279 | 232.79 | + | 1.20 | 3.08 × 10−2 |
| intracellular ribonucleoprotein complex GO:0030529) | 437 | 2 | 14.64 | - | <0.20 | 4.24 × 10−2 |
| ribonucleoprotein complex (GO:1990904) | 438 | 2 | 14.67 | - | <0.20 | 4.12 × 10−2 |
| Unclassified | 6062 | 115 | 203.10 | - | 0.57 | 0.00 × 10 |
| GO molecular function complete | ||||||
| oxidoreductase activity (GO:0016491) | 486 | 38 | 16.28 | + | 2.33 | 3.68 × 10−3 |
| ion binding (GO:0043167) | 3114 | 144 | 104.33 | + | 1.38 | 3.63 × 10−2 |
| Unclassified | 6653 | 155 | 222.9 | - | 0.70 | 0.00 × 10 |
DE turkey genes were matched to the chicken gene reference list for analysis in PANTHER [38]. For each, Gene Ontology category, the number of genes in the reference list and those differentially expressed in the turkey are given. Fold enrichment is the number of DE genes divided by Expected. p-values are as determined by the binomial statistic.