Table 5.
Summary of PANTHER Overrepresentation Test of the 136 unique differentially expressed (DE) genes in livers of Eastern wild turkeys after AFB1 exposure as compared to domesticated turkey.
| GO Biological Process Complete | Gallus gallus—REFLIST Genes (15782) | Observed Turkey Genes | Expected | over/under | Fold Enrichment | p-Value |
|---|---|---|---|---|---|---|
| L-serine biosynthetic process (GO:0006564) | 3 | 3 | 0.01 | + | >100 | 6.60 × 10−4 |
| blood coagulation, fibrin clot formation (GO:0072378) | 5 | 3 | 0.02 | + | >100 | 3.04 × 10−3 |
| plasminogen activation (GO:0031639) | 5 | 3 | 0.02 | + | >100 | 3.04 × 10−3 |
| positive regulation of heterotypic cell-cell adhesion (GO:0034116) | 6 | 3 | 0.02 | + | >100 | 5.24 × 10−3 |
| L-serine metabolic process (GO:0006563) | 6 | 3 | 0.02 | + | >100 | 5.24 × 10−3 |
| zymogen activation (GO:0031638) | 9 | 4 | 0.03 | + | >100 | 1.15 × 10−4 |
| regulation of heterotypic cell-cell adhesion (GO:0034114) | 9 | 3 | 0.03 | + | >100 | 1.76 × 10−2 |
| fibrinolysis (GO:0042730) | 9 | 3 | 0.03 | + | >100 | 1.76 × 10−2 |
| negative regulation of endothelial cell apoptotic process (GO:2000352) | 11 | 3 | 0.03 | + | 87.84 | 3.20 × 10−2 |
| neg regulation of ext apoptotic signaling pathway (GO:1902042) | 11 | 3 | 0.03 | + | 87.84 | 3.20 × 10−2 |
| positive regulation of vasoconstriction (GO:0045907) | 12 | 3 | 0.04 | + | 80.52 | 4.14 × 10−2 |
| positive regulation of peptide hormone secretion (GO:0090277) | 30 | 4 | 0.09 | + | 42.94 | 1.36 × 10−2 |
| cell-matrix adhesion (GO:0007160) | 44 | 5 | 0.14 | + | 36.6 | 1.52 × 10−3 |
| coagulation (GO:0050817) | 65 | 5 | 0.20 | + | 24.78 | 1.02 × 10−2 |
| blood coagulation (GO:0007596) | 65 | 5 | 0.20 | + | 24.78 | 1.02 × 10−2 |
| cell-substrate adhesion (GO:0031589) | 66 | 5 | 0.20 | + | 24.4 | 1.10 × 10−2 |
| hemostasis (GO:0007599) | 66 | 5 | 0.20 | + | 24.4 | 1.10 × 10−2 |
| positive regulation of protein secretion (GO:0050714) | 69 | 5 | 0.21 | + | 23.34 | 1.36 × 10−2 |
| positive regulation of peptide secretion (GO:0002793) | 78 | 5 | 0.24 | + | 20.65 | 2.46 × 10−2 |
| alpha-amino acid metabolic process (GO:1901605) | 104 | 6 | 0.32 | + | 18.58 | 4.71 × 10−3 |
| regulation of response to external stimulus (GO:0032101) | 264 | 8 | 0.82 | + | 9.76 | 7.87 × 10−3 |
| small molecule metabolic process (GO:0044281) | 816 | 13 | 2.53 | + | 5.13 | 4.46 × 10−3 |
| single-organism metabolic process (GO:0044710) | 1775 | 19 | 5.51 | + | 3.45 | 3.17 × 10−3 |
| single-organism process (GO:0044699) | 6214 | 39 | 19.29 | + | 2.02 | 5.62 × 10−5 |
| Unclassified (UNCLASSIFIED) | 6196 | 6 | 19.24 | - | 0.31 | 0.00 × 10 |
DE turkey genes were matched to the chicken gene reference list for analysis in PANTHER [38]. For each, Gene Ontology category, the number of genes in the reference list and those differentially expressed in the turkey are given. Fold enrichment is the number of DE genes divided by Expected. p-values are as determined by the binomial statistic.