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. 2017 Dec 28;9(1):8. doi: 10.3390/genes9010008

Figure 1.

Figure 1

Overexpression of Arabidopsis Radiation sensitive 4 (RAD4) and yellow fluorescent protein (YFP)-RAD4 results in increased tolerance of ultraviolet (UV) radiation. RAD4 levels in (a) 35S:RAD4 and (b) 35S:YFP-RAD4 overexpression lines, relative to control Col-0. The values are normalized relative to the reference gene EF1α. The error bars indicate SE (n = 2). (c–h) UV tolerance in 35S:RAD4 and 35S:YFP-RAD4 overexpression lines. (c,d) Hypocotyl and root growth in dark-incubated seedlings analyzed 3 days after UV treatment, expressed as relative to the untreated controls (n = 10). (e,f) Root growth in light- and dark-incubated seedlings analyzed 2 days after UV treatment, expressed as relative to the untreated controls (n = 10). (g,h) Percentage of undamaged leaves in adult plants after 0, 300, or 600 J m−2 UV treatment followed by dark incubation (n = 6). For c–h, the values are means ± SE, * = p < 0.05 for the overexpression lines versus Col-0 wild type in the same conditions.