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. 2017 Dec 28;9(1):8. doi: 10.3390/genes9010008

Figure 3.

Figure 3

Yeast two-hybrid analysis of the interaction between the RAD4 N-terminus and RAD23B. The RAD4 N-terminal half is sufficient to interact with RAD23B. Five-fold dilutions of the indicated diploid strains were plated on the double drop-out (–leu, –trp) control plate on the left, and the quadruple drop-out (–leu, –trp, –ade, –his) selection medium on the right. The interaction between p53 and T, resulting in growth on the selective medium, is the positive control, whereas Lam and T, which do not interact, are the negative control.