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. 2017 Dec 28;9(1):8. doi: 10.3390/genes9010008

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Yeast two-hybrid analysis of RAD4–HMR interaction. HEMERA (HMR) interacted with both full-length RAD4 and the RAD4 N-terminal half. Five-fold dilutions of the indicated diploid strains were plated on the double drop-out (–leu, –trp) control plate on the left, and the quadruple drop-out (–leu, –trp, –ade, –his) selection medium on the right. The interaction between p53 and T, resulting in growth on the selective medium, is the positive control, whereas Lam and T, which do not interact, are the negative control.