Table 1.

Summary of microRNA screening methodologies.

Year	Initial Screen	Researchers	Type of Cells	Conditions Evaluated in Initial Screen	Reference
Previously identified microRNAs
2015	miR mimics and mir-34 sponge decoy	Kelly et al.	CHO	apoptosis and cell growth	[26]
2015	miR mimics and mir-23 sponge decoy	Kelly et al.	CHO	energy metabolism	[27]
Microarray
2007	human, mouse and rat microRNA arrays	Gammell et al.	CHO	temperature shift	[25]
2009	human and mouse microRNA arrays	Koh et al.	HEK293	3 stages of batch culture	[28]
2011	human microRNA arrays	Barron et al.	CHO	temperature shift	[29]
2011	mouse and rat microRNA arrays	Druz et al.	CHO	apoptosis	[30]
2011	human, mouse and rat microRNA arrays	Lin et al.	CHO	producing lines compared to parental and MTX amplification	[31]
2014	cross-species microRNA and mRNA arrays	Maccani et al.	CHO	high producing cell lines compared to low producing cell lines	[32]
2016	human for HELA, mouse, rat and human for CHO microRNA arrays	Emmerling et al.	HELA and CHO	mild hypothermia	[33]
2016	human, mouse, rat, viral microRNAs	Klanert et al.	CHO	growth rate in multiple cell lines	[34]
microRNA screen
2013	human microRNA library	Strotbek et al.	CHO	IgG	[35]
2014	murine microRNA library	Fischer et al.	CHO	SEAP	[36]
2015	human microRNA library	Xiao et al.	HEK293	neurotensin receptor	[37]
2017	human microRNA library	Meyer et al.	HEK293	antibody	[38]
Next Generation Sequencing
2011	small RNA transcriptome	Hackl et al.	CHO	identified conserved and novel CHO microRNAs	[39]
2012	microRNA	Jadhav et al.	CHO	effects of overexpressing microRNA	[40]
2014	microRNA	Loh et al.	CHO	looking at profile of different expression level cultures	[41]
2016	microRNA and mRNA	Pfizenmaier et al.	CHO	osmotic shift	[42]
2016	microRNA	Stiefel et al.	CHO	biphasic fed batch cultivation of high low and non-producing CHO lines with mild hypothermia	[43]