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. 2018 Jan 11;9(1):30. doi: 10.3390/genes9010030

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression patterns of AvTCTP in various tissues. (a) Non-quantitative RT-PCR performed to define the tissue specific expression pattern. Total RNA was extracted from different anatomical districts of the sea anemone: tentacles (T), pharynx (P), mesenterial filaments (M) basal disk (D), body column (B). (b) RT-qPCR of AvTCTP mRNA in the same tissues. Transcript levels in tentacles, pharynx, basal disk and body column were normalised to that of mesenterial filaments. RCC2 and COP-γ were used as housekeeping genes. The results are represented as means ± SD (n = 3). Statistical analysis by one-way ANOVA with Tukey’s post-test. * p < 0.01.