Table 1.

Working method for NO metabolites (NOx) determination.

Reagents (μL)	Test	Control
Supernatant	360	360
PBS	310	540
l-Arginine 1	60	-
FAD 2	60	-
NADPH 3	10	-
37 °C, 60 min
Cadmium chloride	0.5 g/mL	0.5 g/mL
25 °C, 30 min
Griess reagent	1000	1000

¹ 1.3 mg l-arginine/10 mL solution; ² 0.6 mg FAD/10 mL solution; ³ 12.5 mg NADPH/1 mL solution. PBS, phosphate-buffered saline.