Table 1.
Primer sequences used in quantitative real-time PCR.
| Gene | Primers | Sequence (5′ to 3′) |
|---|---|---|
| SREBP-1c | Forward | TGCATTTTCTGACACGCTTC |
| Reverse | CCAAGCTGTACAGGCTCTCC | |
| FAS | Forward | CCCCTGATGAAGAAGGATCA |
| Reverse | ACTCCACAGGTGGGAACAAG | |
| SCD-1 | Forward | TTGGAGAAGCGGTGGATAAC |
| Reverse | AAAAATCCCACCCAATCACA | |
| CD36 | Forward | TGGAACAGAGGCTGACAACT |
| Reverse | TTGATTTTTGATAGATATGGG | |
| PPAR-α | Forward | ACGATTCGACTCAAGCTGGT |
| Reverse | GTTGTGTGACATCCCGACAG | |
| CPT-1 | Forward | CCT CCG TAG CTG ACT CGG TA |
| Reverse | GGA GTG ACC GTG AAC TGA AA | |
| β-actin | Forward | CTCTTCCAGCCTTCCTTCCT |
| Reverse | AGCACTGTGTTGGCGTACAG |
SREBP-1c, sterol regulatory element binding protein1-c; FAS, fatty acid synthase; SCD-1, stearoyl-CoA desaturase-1; CD36, cluster of differentiation 36; PPAR-α, peroxisome proliferator-activated receptor-alpha; CPT-1, carnitine palmitoyltransferase-1.