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. 2018 Jan 15;10(1):91. doi: 10.3390/nu10010091

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Involvement of Akt signalling in deoxyschizandrin-induced cell cycle arrest in A2780 cells: (A) A2780 cells were transfected with Akt expression vector and were treated with deoxyschizandrin (30 µM) for 48 h. MTT assay was performed to measure the cell viability in A2780 cells. The values represent the mean ± SD of results from three independent experiments. # p < 0.05 vs. the control group; * p < 0.05 vs. the treated group transfected with empty vector; (B) A2780 cells were treated with 5 mM of N-acetyl-l-cystein (NAC) and deoxyschizandrin (30 µM) for 8 h. Western blot analysis was performed to measure the phosphorylation of Akt in A2780 cells. β-Actin was used as an internal control. Band images are representatives of three individual experiments and the results of densitometry represent the mean ± SD of three independent experiments. # p < 0.05 vs. the control group; * p < 0.05 vs. the treated group.

Involvement of Akt signalling in deoxyschizandrin-induced cell cycle arrest in A2780 cells: (A) A2780 cells were transfected with Akt expression vector and were treated with deoxyschizandrin (30 µM) for 48 h. MTT assay was performed to measure the cell viability in A2780 cells. The values represent the mean ± SD of results from three independent experiments. # p < 0.05 vs. the control group; * p < 0.05 vs. the treated group transfected with empty vector; (B) A2780 cells were treated with 5 mM of N-acetyl-l-cystein (NAC) and deoxyschizandrin (30 µM) for 8 h. Western blot analysis was performed to measure the phosphorylation of Akt in A2780 cells. β-Actin was used as an internal control. Band images are representatives of three individual experiments and the results of densitometry represent the mean ± SD of three independent experiments. # p < 0.05 vs. the control group; * p < 0.05 vs. the treated group.