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. 2018 Jan 22;10(1):110. doi: 10.3390/nu10010110

Figure 5.

Figure 5

Effect of the Stauntonia hexaphylla fruit (SHF) extract on lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) protein, cyclooxygenase-2 (COX-2) protein expression and cyclooxygenase (COX) enzyme activity in RAW 264.7 cells. Cells were treated with 50, 100 and 200 µg/mL of the SHF extract and later treated with LPS (1 µg/mL) for 18 h. (A) iNOS protein levels were assessed by Western blotting; (B) COX-2 protein levels were assessed by Western blotting; (C) COX enzyme activity was assessed by a fluorescent assay. Data are presented as means ± SD. * p < 0.05 and ** p < 0.01 compared to the LPS-stimulated group (ANOVA followed Dunnett’s test).