Figure 7.

Effect of the Stauntonia hexaphylla fruit (SHF) extract on nuclear factor (NF)-κB translocation in RAW 264.7 cells. Cells were treated with 50, 100 and 200 µg/mL of the SHF extract and later treated with LPS (1 µg/mL) for 18 h. After treatment, nuclear and cytosolic extracts were prepared, and equal amounts of proteins were separated by Western blot analyses. (A) NF-κB protein expression was determined using an antibody specific for p65; (B) Samples were treated as described for Figure 4, and phosphorylation of inhibitor of κB (IκB)α was analyzed by Western blotting.