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. 2018 Jan 31;9:20. doi: 10.1186/s13287-017-0745-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Confirmation of hPMSC characteristics by cell surface marker staining and differentiation capability of cells. Surface markers of hPMSCs measured by flow cytometry. a Green line in each histogram represents isotype control staining; specific expression of indicated cell surface marker presented as blue histograms. b–d Cells cultured under various conditions to differentiate to osteoblast or adipogenesis cells. b Normal hPMSCs show fibroblast-like morphology. c Osteoblasts confirmed by Alizarin Red staining; dark red staining shows calcium deposition. d Adipogenesis confirmed by accumulation of neutral lipid vacuoles inside cells with Oil Red O stain. Scale bar: 200 μm. Magnification × 200