Fig. 6.

miR-185 regulates β-catenin through ITGB5. a–b miR-185 was introduced into MHCC-97 L and Huh-7 cells. Cell lysates were analysed using the indicated antibodies (a). The β-catenin protein levels were quantitatively measured (b). c The inhibition of b-catenin/TCF/LEF-mediated transcription by miR-185 in MHCC-97 L and Huh-7 cells was monitored. Data represent the mean ± SD of three independent experiments. **p < 0.01vs. control. d–f miR-185 inhibitor was transfected into MHCC-97 L and Huh-7 cells with or without ITGB5 knockdown. Cell lysates were examined by western blotting using the indicated antibodies (d). The β-catenin protein levels were quantitatively measured (f). The effect of miR-185 inhibitor on b-catenin/TCF/LEF-mediated transcription was measured. Data represent the mean ± SD of three independent experiments. **p < 0.01 and ***p < 0.001 vs. control. g–h miR-185 inhibitor was transfected into MHCC-97 L and Huh-7 with or without ITGB5 or β-catenin knockdown. Cell proliferation was examined by colony formation assay. Data represent the mean ± SD of three independent experiments. ***p < 0.001 vs. control. i–j Cell migration was analysed by transwell assay. Data represent the mean ± SD of three independent experiments. **p < 0.01 vs. control