Table 2.
Genes and primers used in qRT-PCR assay
| Target gene | Gene function | Primer sequences (5’-3’) | Amplification efficiencya | Tm (°C) |
|---|---|---|---|---|
| DELLA | SAR signalling | F: GTCTTCTAATTCAAACCA | 1.926 ± 0.027 | 53 |
| R: ATATCTGTTTACCCAAGTAA | ||||
| RBP-hnRNP | Transcriptional factor | F: GAGAAAGATATTTGTTGGAG | 1.947 ± 0.024 | 51 |
| R: TGATCGTACATTACTACAACA | ||||
| PGIP | Anti fungal compound | F: TGAAGGTGATGCTTCTATGCT | 2.013 ± 0.026 | 53 |
| R: GACTCACATCCAACGTTGCT | ||||
| PR2 | Anti fungal compound | F: GGCATGCTGGGAAACAATCT | 2.018 ± 0.023 | 52 |
| R: TGGCACACCTAACATGAGCT | ||||
| PR10 | Anti fungal compound | F: TGGCACTTCTGCTGTTAGATGGAC | 2.017 ± 0.083 | 50 |
| R: GGTAATCCATCCAGCCATTTGGAG | ||||
| PP2A | Reference gene | F: GCCTCATTTGCAGCTGGTTT | 2.002 ± 0.025 | 53 |
| R: TACTCTCGTTCTAGGGTCCT |
aMean and standard deviation of the amplification efficiency for each gene were calculated from the coefficients of linear regression models fitted to each reaction (value of 2 equivalents to 100% efficiency, see [43])