Table 2.
Characterization of formulations developed for the study. Showing adsorption percentages after 2 h and 24 h for AlOH formulations; Zeta potential and pH for SWE formulations; particle size and pH for QS21-Liposome
| AlOH | SWE | QS21-Liposomes | ||||
|---|---|---|---|---|---|---|
| Antigen | Time (h) | Adsorption (%) | Size (nm) | pH | Size (nm) | pH |
| AMA1 | 2 | > 99.9a | 133.8 ± 1.2 | 6.27 | 130.8 ± 0.4 | 6.88 |
| 24 | > 99.9a | 133.9 ± 1.0 | 6.29 | 121.2 ± 1.6 | 6.98 | |
| HBsAg | 2 | > 99.9a | 133.5 ± 1.5 | 6.26 | 134.0 ± 1.3 | 7.18 |
| 24 | > 99.9a | 133.6 ± 1.1 | 6.26 | 126.7 ± 0.1 | 7.14 | |
| Ag85A | 2 | > 98.0b | 133.2 ± 1.1 | 6.48 | 139.6 ± 3.9 | 7.31 |
| 24 | > 98.0b | 134.3 ± 0.9 | 6.53 | 138.9 ± 1.2 | 7.37 | |
| Control | 2 | N/A | 134.4 ± 2.3 | 6.50 | 132.1 ± 2.6 | 7.36 |
| 24 | N/A | 134.3 ± 1.0 | 6.51 | 111.7 ± 12.0 | 7.36 | |
abased on ELISA results (limit of quantification was 12.5 ng/mL for AMA1 and 5 ng/mL for HBsAg)
bbased on mBCA™ (limit of quantification was 2 μg/mL)