Fig. 1.

Construction of RDRP bait plasmids and testing of RDRP expression, toxicity, and autoactivation in yeast cells. a Agarose gel electrophoresis analysis of the bait plasmids pGBKT7-RDRP and pGBKT7. M: DNA marker; Lane 1: bait plasmid pGBKT7-RDRP digested with NdeI and SalI; Lane 2: pGBKT7 vector. b Immunoblot analysis of total proteins in the yeast strain Y187 transformed with the indicated plasmids. Lane 1: pGBKT7-RDRP; Lane 2: pGBKT7 vector. c Determination of the autoactivation and toxicity of RDRP bait in yeast cells. Yeast strain Y187 was transformed with pGBKT7-RDRP (upper) or pGBKT7 (lower) and grown on SD/-Trp and SD/-Trp/-X-α-gal plates. Lack of autoactivation is indicated by white colonies (or pink colonies) on SD/-Trp and SD/-Trp/-X-α-gal plates. Toxic bait is indicated by colonies significantly smaller than those containing the pGBKT7 empty vector