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Fixative combinations used in this study. All fixative combinations were performed in triplicate on 16HBE14o- cultured cells. Immunocytochemistry was performed on cells as detailed
| Pre-extraction | Fixation | Permeabilization |
|---|---|---|
| – | 4% Paraformaldehyde | – |
| 0.2% Triton X-100 | 4% Paraformaldehyde | – |
| – | 4% Paraformaldehyde | 0.1% Saponin |
| – | 4% Paraformaldehyde + Acetone | – |
| – | 100% Methanol | – |
| 0.2% Triton X-100 | 100% Methanol | – |
| – | 100% Methanol | 0.1% Saponin |
| – | 1:1 Methanol:Acetone | – |
| – | 100% Acetone | – |
