Table 8.
Composition of Tris-buffered SBF 27.
| Reagent | Obtained by | Art. No. | Quantity (g) |
|---|---|---|---|
| Sodium chloride (NaCl) | Roth | 3957.3 | 3.274 |
| Sodium hydrogen carbonate (NaHCO3) | Roth | 6885.2 | 1.134 |
| Potassium chloride (KCl) | Sigma | P5405 | 0.187 |
| di-sodium hydrogen phosphate dihydrate (Na2HPO4·2H2O) | Merck | 6580.0500 | 0.089 |
| magnesium chloride hexahydrate (MgCl2·6H2O) | Sigma | M8266 | 0.071 |
| calcium chloride dihydrate (CaCl2·2H2O) | Sigma | C7902 | 0.184 |
| sodium sulphate (Na2SO4) | Sigma-Aldrich | 746363 | 0.0355 |
| Tris ((CH2OH)3CNH2) | Serva | 37180 | 3.0285 |
| 1M HCl | Roth | CN63.1 | Until pH 7.4 |