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I3A induces intrinsic apoptosis pathway. (A, B) Western blot analysis of apoptosis related proteins from A2058 and HT144 cells treated with or without I3A. (C) MTT cell viability assay for A2058 and HT144 cells treated with or without I3A. Cell viability is represented as percent of control from experimental triplicates. I3A-1 – ingenol-3-angelate 1 μM; I3A-5 – ingenol-3-angelate 5 μM; z-VAD-fmk concentration 5 μM; * P<0.05 vs. control.
