Skip to main content
. 2018 Jan 25;24:486–502. doi: 10.12659/MSM.906049

Figure 4.

Figure 4

I3A downregulates NF-κB signaling. (A, B, D) Western blot analysis of proteins from A2058 and HT144 cells treated with or without I3A. (C) Quantitative real-time PCR analysis of NF-κB, iNOS, and Cox-2 genes from A2058 and HT144 cells treated with or without I3A. The data represented are fold changes (mean ±SD) of genes expression in I3A-treated samples vs. control (one-fold) from experimental triplicates. (E) MTT cell viability assay for A2058 and HT144 cells treated with or without I3A. Cell viability is represented as percent of control from experimental triplicates. I3A – ingenol-3-angelate; Cyt – cytosolic fraction; Nuc – nuclear fraction; prostratin concentration 100 nmol; * P<0.05 vs. control; ** P<0.05 vs. prostratin group.