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Effect of ghrelin on energy expenditure in DIO mice. Energy expenditure in 2-week CD- (A) or HFD-fed mice (B), and 4-week CD- (C) or HFD-fed mice (D) receiving ghrelin. Values are means ± s.e.m. n = 4–12. *P < 0.05, **P < 0.01, ***P < 0.001.
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