Figure 6.

Impact of quantitative and qualitative differences in N-glycosylation on proteolysis of human CBG. (A) FPLC-Purified human CBG and CBG N238 produced in CHO-S, Lec1, or Lec2 cells were incubated with neutrophil elastase (0.1 µg for 10 min at 37°C), chymotrypsin (1 µg for 10 min at 37°C), or P. aeruginosa media (5 µL for 3 h at 37°C) containing LasB and subjected to Western blotting. Reductions (~5–10 kDa) in molecular size were observed after incubation with proteases. (B and C) Western blots of human CBG N238 after limited incubation times with different amounts of chymotrypsin or LasB. Positions of molecular size markers (kDa) are indicated.