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. 2018 Feb;4(1):a002303. doi: 10.1101/mcs.a002303

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© 2018 Berland et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial License, which permits reuse and redistribution, except for commercial purposes, provided that the original author and source are credited.

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Figure 2. — The relative unit water permeability of AQP4-wt and AQP4-S111T expressed in oocytes. (A) Representative volume traces from an uninjected oocyte (labeled C) and oocytes expressing AQP4-wt or AQP4-S111T challenged with a hyperosmotic gradient of +20 mOsm (indicated by a black bar). (B) The water permeabilities are given for uninjected control oocytes (labeled “C”), AQP4-wt-, and AQP4-S111T-expressing oocytes for one representative experiment (n = 4 oocytes, error bars given as ±SD, statistical significance above the histograms refers to comparison to uninjected oocytes). (C) A representative western blot of biotinylated oocyte plasma membranes from one experiment (n = 4 oocytes in each lane). (D) Quantification of the representative western blot in C. (E) The relative unit water permeability was obtained for each experiment by division of the AQP4-mediated water permeabilities from B with plasma membrane AQP4 abundance (D), normalized to AQP4-wt and summarized across individual experiments (n = 3, error bars given as ±SEM). Water permeabilities were compared using one-way ANOVA with Dunnett's multiple comparison post hoc test and the relative unit water permeabilities were compared using Student's paired t-test. ***, P < 0.001; ns, not significant.