Figure 2.

Deletion of GRP94 in Pdx1⁺ cells leads to pancreas hypoplasia and reduced β-cell numbers during embryonic development. (A, B) Expression of LacZ (dark blue) in CTR and KO embryos at (A) E10.5 and (B) E18.5. Circled areas in A and B indicate pancreatic tissues, and black arrows in A point to LacZ expression in brain. (C) Hematoxylin and eosin staining of pancreas sections from CTR and KO mice. (D) Quantification of pancreatic area in CTR and KO mice at day 18.5. (E) Micrographs of pancreases and connected intestine from CTR and KO mice. (F) Representative immunohistochemical staining of glucagon (Glu) for α cells (green), insulin (Ins) for β cells (red), and somatostatin (Soma) for δ cells (gray) in pancreases from CTR and KO mice at E14.5, E16.5, and E18.5. Nuclei are stained blue. Scale bar = 50 μm. *P < 0.05, Student t test.