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. 2017 Dec 20;159(2):1062–1073. doi: 10.1210/en.2017-00685

Figure 5.

Figure 5.

Deletion of GRP94 leads to more cell death and ER structural changes in KO mice compared with CTR. (A) Immunohistochemical staining and (B) quantification of TUNEL+ (green) cells among Pdx1+ (red) cells in embryos from CTR (n = 4) and KO (n = 5) mice at E12.5. Nuclei are stained blue. Scale bar = 50 μm. (C) Cleaved caspase-3 (c-Cas-3) staining in pancreatic islets from CTR (n =3) and KO (n = 3) mice at E18.5. GRP94 (gray), glucagon (green), and c-Cas-3 (red). Nuclei are stained blue. White arrows point to c-Cas-3+ cells. Scale bar = 50 μm. (D) Transmission electron microscopy of pancreatic β cells from CTR and KO mice at E16.5. Islets are identified by the presence of insulin-containing granules with a typical electron-dense core, readily seen at higher magnification (right panels). Mt, mitochondria; N, nucleus. Scale bar: left = 2 μm and right = 500 nm. *P < 0.05, Student t test.