Figure 1. Miro1 and Miro2 function is critical during early embryonic development.

• A–C
  Miro1^het/Miro2^KO embryos (with only one copy of Miro1) are not viable beyond day E12.5 of gestation as opposed to Miro2^KO embryos (with two copies of Miro1). At E16.5 (A) and E14.5 (B), all embryos that were identified post hoc as heterozygotes for Miro1 and knock out for Miro2 were found in advanced state of reabsorption. At E12.5 (C), half of the embryos of this genotype were found to be indistinguishable from WT control animals. A viable embryo was selected as a control animal for comparison. See also Table EV1.
• D, E
  Miro^DKO embryos were found to be not viable from E10.5. (D) At this stage, they were very small and presented malformations and oedema in head and viscera compared with viable littermates. Neural tube closure was incomplete (arrowheads). (E) Further observation showed that Miro^DKO embryos at E10.5 failed in generating the vasculature that irrigates the yolk sac (arrows).
• F
  Western blot analysis of E10.5 heads (or whole body for Miro^DKO embryos) showing the specificity of the different bands recognised by the antibody (anti‐Miro1 from Atlas) and the complete depletion of Miro1 and Miro2 proteins in Miro^DKO embryos.
• G
  Western blot analysis of brains from E12.5 embryos showing that the protein levels correlate with the genetic dosage of Miro1 and Miro2. Quantification of Miro1 and Miro2 protein levels provided in Fig EV1.

Source data are available online for this figure.