Figure 4. Miro proteins are not required for TRAK/kinesin‐dependent anterograde movement.

• A–H
  Effect of TRAK1 and TRAK2 with and without KIF5C overexpression on mitochondrial redistribution in WT (A, C, D, and E) or Miro^DKO (B, F, G and H) cell lines. Reference cells generated by projection of 10 WT (A) or Miro^DKO (B) cells with the same transfection combination. An inset in each cell is shown magnified below to better show the occupancy of mitochondria in the tips of the triangular cell. MPM from WT cells (C and D) or Miro^DKO cells (F and G) overexpressing TRAK1GFP (C and F) or TRAK2GFP (D and G), respectively. (E and H) Mito⁹⁵ values calculated from the above experimental conditions in WT (E) and Miro^DKO (H) cell lines. Data obtained from three independent experiments (n = number of cells; in WT: control 56; TRAK1GFP: 55; TRAK1 + KIF5C 55; TRAK2GFP 48; TRAK2GFP + KIF5C 46; and in Miro^DKO: control 60; TRAK1GFP 61; TRAK1 + KIF5C 56; TRAK2GFP 62; TRAK2GFP + KIF5C 54; ANOVA‐NK). Error bars represent s.e.m. Statistical significance: *P < 0.05 and ***P < 0.001.