Table 2.

The Amount of Reads Gained from Small RNA Sequencing of Anemonia viridis

Individuals	Raw Readsa	Filtered Small RNA Reads (≥18 nt)b	% Reads Aligned to the Genome (≥18 nt)	Filtered Small RNA Reads (≥23 nt)b	Reads Aligned to Genome (≥23 nt)	Putative piRNA Reads Aligned to Genomec	% Putative piRNAs Aligned to Genome
pH 7.6–1	16,111,517	12,117,933	88.5	9,831,771	7,681,304	6,643,549	86.5
pH 7.6–2	12,492,090	11,175,766	89.5	9,465,543	7,721,730	6,795,032	88.0
pH 7.6–3	13,794,761	10,609,870	89.1	8,921,147	7,238,217	6,310,624	87.2
pH 7.9–1	18,815,344	15,837,619	91.5	13,235,131	11,175,365	10,001,436	89.5
pH 7.9–2	20,094,253	17,369,794	90.0	16,247,704	13,837,596	11,960,594	86.4
pH 7.9–3	16,794,089	15,567,040	89.3	14,958,985	12,687,556	11,353,938	89.5
pH 8.2–1	13,066,319	12,109,753	88.0	11,317,833	9,184,500	8,163,612	88.9
pH 8.2–2	17,379,705	10,309,153	90.3	8,124,195	6,661,625	5,543,870	83.2
pH 8.2–3	13,492,380	11,225,436	90.3	8,769,865	7,189,261	6,271,324	87.2

^aSmall RNA libraries from each individual were barcoded, pooled, and sequencing was performed on three lanes of a SOLiD™ 6-Lane FlowChip using the SOLiD 5500xl sequencer. The amount of sequences presented here is the sum of raw reads from the three lanes.

^bThese reads had adapter removed and were quality filtered. They differ only according to the size filtering.

^cReads (≥23 nt) aligned to the reference genome and filtered for piRNA sequence signatures (1 U and 10 A).