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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Science. 2017 Oct 25;358(6366):1019–1027. doi: 10.1126/science.aaq0180

Figure 3. Measuring sequence flexibility for RNA editing by REPAIRv1.

Figure 3

A) Schematic of screen for determining Protospacer Flanking Site (PFS) preferences of RNA editing by REPAIRv1. A randomized PFS sequence is cloned 5’ to a target site for REPAIR editing. Following exposure to REPAIR, deep sequencing of reverse transcribed RNA from the target site and PFS is used to associate edited reads with PFS sequences.

B) Distributions of RNA editing efficiencies for all 4-N PFS combinations at two different editing sites

F) Quantification of the percent editing of REPAIRv1 at Cluc W85 across all possible 3 base motifs. Values represent mean +/− S.E.M. Non-targeting guide is the same as in Fig. 2C.

C) Heatmap of 5’ and 3’ base preferences of RNA editing at Cluc W85 for all possible 3 base motifs.