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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Science. 2017 Oct 25;358(6366):1019–1027. doi: 10.1126/science.aaq0180

Figure 5. Characterizing specificity of REPAIRv1.

Figure 5

A) Schematic of KRAS target site and guide design.

B) Quantification of percent A to I editing for tiled KRAS-targeting guides. Editing percentages are shown for the on-target (blue triangle) and neighboring adenosine sites. For each guide, the region of duplex RNA is outlined in red. Values represent mean +/− S.E.M.

C) Transcriptome-wide sites of significant RNA editing by REPAIRv1 (150ng REPAIR vector transfected) with Cluc targeting guide. The on-target site Cluc site (254 A>I) is highlighted in orange.

D) Transcriptome-wide sites of significant RNA editing by REPAIRv1 (150ng REPAIR vector transfected) with non-targeting guide. Non-targeting guide is the same as in Fig. 2C.