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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Transfusion. 2015 Dec 12;56(4):980–993. doi: 10.1111/trf.13442

Fig. 1.

Fig. 1

(A) Overview of the metabolomics workflow. (B) General sensitivity and specificity attributes for NMR and MS technologies. Targeted MS-based approaches such as multiple reaction monitoring (MRM) increase sensitivity of the assays, at the expense of the total number of compounds monitored in one analysis. (C and D) Representative three-dimensional and two-dimensional spectrum of a routine 12-minute run of RBC extracts. (E) Representative schematic of metabolite identity assignment on the basis of accurate intact mass, chromatographic retention times, isotopic patterns, and the seven golden rules for the heuristic determination of compound identifications on the basis of MS profiles.