Fig 4. Effects of oral administration of Euglena gracilis Z and paramylon on serum IgG titer in collagen-induced arthritis mouse model.

After terminating the test substance administration, laparotomies were performed under inhalation anesthesia with isoflurane, and blood samples were collected from the abdominal vena cava. After the blood collection, the animals were euthanized by exsanguination, and then the inguinal lymph nodes and knee joints were harvested. The serum was separated from the blood samples by centrifugation to quantify the IgG levels using an enzyme-linked immunosorbent assay (ELISA). Value are means ± standard deviation (SD) of five mice.