Table 2. Intra assay variability of the platelet function test and beads.
| Intra-assay variability | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Donor 1 (n = 10) | Donor 2 (n = 10) | Donor 3 (n = 10) | Donor 4 (n = 10) | MEAN %CV | |||||
| Mean±SD | %CV | Mean±SD | %CV | Mean±SD | %CV | Mean±SD | %CV | ||
| αIIbβ3 activation | |||||||||
| TRAP | 1050±38 | 3.6 | 1738±71 | 4.1 | 972±51 | 5.2 | 1196±84 | 7.0 | 5.0 |
| CRP | 8475±100 | 1.2 | 10384±568 | 5.5 | 7162±195 | 2.7 | 8297±374 | 4.5 | 3.5 |
| ADP | 8077±984 | 12.2 | 6243±280 | 4.5 | 4351±144 | 3.3 | 3912±161 | 4.1 | 6.0 |
| P-selectin expression | |||||||||
| TRAP | 8804±340 | 3.9 | 9621±232 | 2.4 | 7965±377 | 4.7 | 7603±363 | 4.8 | 3.9 |
| CRP | 8995±227 | 2.5 | 10205±244 | 2.4 | 8246±382 | 4.6 | 8308±190 | 2.3 | 3.0 |
| ADP | 5219±1560 | 29.9 | 1048±155 | 14.8 | 923±272 | 29.4 | 599±46 | 7.7 | 20.4 |
| Positive control beads | |||||||||
| PAC-1-FITC | 18209±134 | 0.7 | 21047±573 | 2.7 | 19795±468 | 2.4 | 19584±331 | 1.7 | 1.9 |
| aCD62P-PE | 39510±692 | 1.8 | 46287±1833 | 4.0 | 43513±732 | 1.7 | 40992±1361 | 3.3 | 2.7 |
Due to the need of fresh blood, a true inter-assay CV determined on consecutive days could not be assessed.