Table 1. Information on the studied specimens.

Taxon	Storage method	Collecting data	Age	Number of specimens
Pulex irritans (Siphonaptera)	Frozen material	Brazil, São Paulo, Bom Jesus dos Perdões (23.135 S 46.4656W), donated by Dr. Arlei Marcili in 2016.	Less than 1 year	7
Phlebotomus sp.(Diptera: Psychodidae)	Frozen material	Brazil, São Paulo, Bom Jesus dos Perdões (23.135 S 46.4656W), donated by Dr. Arlei Marcili in 2016.	Less than 1 year	11
Aedes aegypti (Diptera: Culicidae)	Frozen material	Strain RED specimens, donated by Dr. Margareth de Lara Capurro Guimarães in 2016.	Less than 1 year	5
Geranomyia sp. (Diptera: Limoniidae)	Ethanol 70°	Brazil, Santa Catarina, Joaçaba (27.1606S 51.5225W), Pinho LC and Muller GA, October 2012.	5 years	2
Chrysopilus balbii (Diptera: Rhagionidae)	Ethanol 70°	Brazil, São Paulo, Salesópolis (23.6500S 45.7333W), Papavero N, May 1968	14 years	1
Aphrophila chilena (Diptera: Limoniidae)	Pinned	Chile, Araucanía, Angol [37.1667S 72.1000W], Bullock DS, November 1929	87 years	1