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. 2018 Feb 1;13(2):e0192447. doi: 10.1371/journal.pone.0192447

Fig 4. mRNA expression of carbohydrate-responsive element-binding protein (ChREBP) target genes in HepG2 cells following CLA treatment.

Fig 4

HepG2 cells were incubated with bovine serum albumin (BSA) or with 10 μM of linoleic acid (LA), cis-9,trans-11 (c9t11) CLA, cis-9,cis-11 (c9c11) CLA or trans-11,trans-13 (t11t13) CLA for 24 h. The mRNA expression of liver pyruvate kinase (L-PK) and glucose-6 phosphatase (G6Pase) were measured by qPCR and expressed as relative expression. Data are the means ± SEM of 2 independent experiments (n = 6 to 12). Data with different superscript letters are significantly different (p ≤ 0.05) according to the post-hoc ANOVA statistical analysis.