Fig 2. Growth of S. aureus upon BCAA depletion.

A) WT USA300 was pre-grown in complete CDM to mid-exponential phase and then sub-cultured into either complete CDM or CDM with BCAAs omitted, as indicated. B) USA300 with a transposon insertion in codY (codY::ϕNΣ) was pre-grown in complete CDM to mid-exponential phase, and then sub-cultured into either complete CDM or CDM with BCAAs omitted, as indicated. C) Cells recovered from the CDM with no Val in panel A were plated. A single colony was selected (Val^Sup (abbreviated Val^S) —filled symbols) and subjected to growth in complete CDM and CDM with no Val. Growth was compared to the parental WT strain (open symbols) in the same conditions. Data are the mean +/- SD of three biological replicates.