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. 2018 Feb 1;13(2):e0192230. doi: 10.1371/journal.pone.0192230

Fig 4. Impaired class-switching in neonatal B cells.

Fig 4

Sort-purified adult and neonatal B cell subsets (transitional 1 & 2 B cells: T1 & T2; naïve mature B cells: N) were stimulated with CpG alone or in combination with IL-4, IL-21, anti-CD40, and BAFF (SC) for 8 days. After this time, cells were harvested and immunoglobulin levels for (A) IgM, (B) IgG, and (C) IgA, were determined in the supernatant by ELISA; (n = 5). (D) Surface IgG expression in sorted and stimulated (5 d; ctrl = not stimulated, with CpG or SC alone; AB: n≥3, CB: n = 5) B cells were determined by flow cytometry. Students t-test; * P<0.05, ** P<0.01, *** P<0.0001.