Table 4. Candidate genes expression stability value (M) of single and best combination of two genes estimated by NormFinder algorithm in different datasets.

For dataset symbols, refer to Table 3.

Sample set	Candidate reference gene														Best combination of two genes
	AfEF1a		AfGAPDH1		AfGAPDH2		AfTBP2		AfTUA1		AfUBC1		AfUBC2
	M	Rank	M	Rank	M	Rank	M	Rank	M	Rank	M	Rank	M	Rank		M
Total	0.360	4	0.364	5	0.432	7	0.327	3	0.411	6	0.286	1	0.314	2	AfGAPDH1, AfGAPDH2	0.155
NPC	0.387	5	0.373	4	0.414	6	0.273	1	0.423	7	0.312	3	0.305	2	AfEF1a, AfGAPDH1	0.121
NPC+SOT	0.340	6	0.291	4	0.330	5	0.278	3	0.353	7	0.276	2	0.255	1	AfEF1a, AfGAPDH1	0.136
NC+S	0.611	6	0.440	5	0.358	3	0.304	1	0.949	7	0.381	4	0.346	2	AfGAPDH1, AfUBC1	0.143
NC+SOT	0.354	6	0.348	5	0.346	4	0.324	3	0.370	7	0.240	2	0.221	1	AfEF1a, AfTBP	0.094
PC+KG	0.291	2	0.339	5	0.547	7	0.217	1	0.311	4	0.297	3	0.360	6	AfTUA1, AfUBC1	0.107