Table 5. Ranking of candidate reference genes according to the BestKeeper analysis of their expression stability.
For dataset symbols, refer to Table 3.
| Sample set | Candidate reference genes | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AfEF1A | AfGAPDH1 | AfGAPDH2 | AfTBP2 | AfTUA1 | AfUBC1 | AfUBC2 | |||||||||
| SV | Rank | SV | Rank | SV | Rank | SV | Rank | SV | Rank | SV | Rank | SV | Rank | ||
| Total | SD | 0.99 | 5 | 0.46 | 1 | 1.25 | 7 | 0.59 | 4 | 1.22 | 6 | 0.58 | 3 | 0.47 | 2 |
| CV | 4.89 | 6 | 2.19 | 2 | 5.56 | 7 | 2.21 | 3 | 4.70 | 5 | 2.41 | 4 | 1.98 | 1 | |
| r | 0.881 | 4 | 0.793 | 7 | 0.930 | 2 | 0.820 | 6 | 0.966 | 1 | 0.882 | 3 | 0.839 | 5 | |
| Mean of sub-ranks | 5.00 | 3.33* | 5.33 | 4.00 | 4.33 | 3.33* | 2.67 | ||||||||
| BK ranking | 5 | 2 | 6 | 3 | 4 | 2 | 1 | ||||||||
| NPC | SD | 1.06 | 3 | 0.39 | 1 | 1.12 | 4 | 0.69 | 2 | 1.20 | 5 | 0.39 | 1 | 0.39 | 1 |
| CV | 5.18 | 7 | 1.82 | 3 | 4.82 | 6 | 2.52 | 4 | 4.44 | 5 | 1.64 | 2 | 1.60 | 1 | |
| r | 0.979 | 1 | 0.702 | 7 | 0.962 | 2 | 0.882 | 5 | 0.929 | 3 | 0.920 | 4 | 0.891 | 6 | |
| Mean of sub-ranks | 3.67* | 3.67* | 4.00 | 3.67* | 4.33 | 2.33 | 2.67 | ||||||||
| BK ranking | 3 | 3 | 4 | 3 | 5 | 1 | 2 | ||||||||
| NPC + SOT | SD | 0.76 | 6 | 0.38 | 3.00 | 0.75 | 5 | 0.56 | 4 | 0.82 | 7 | 0.35 | 2 | 0.33 | 1 |
| CV | 3.71 | 7 | 1.77 | 3.00 | 3.24 | 6 | 2.05 | 4 | 3.07 | 5 | 1.45 | 2 | 1.37 | 1 | |
| r | 0.929 | 2 | 0.614 | 7.000 | 0.941 | 1 | 0.796 | 5 | 0.906 | 3 | 0.735 | 6 | 0.820 | 4 | |
| Mean of sub-ranks | 4.67 | 4.33* | 4.33* | 4.33* | 5.00 | 3.33 | 2.00 | ||||||||
| BK ranking | 4 | 3 | 3 | 3 | 5 | 2 | 1 | ||||||||
| NC + S | SD | 1.28 | 4 | 1.29 | 5.00 | 1.49 | 6 | 0.91 | 1 | 2.67 | 7 | 1.22 | 3 | 1.14 | 2 |
| CV | 6.61 | 5 | 6.23 | 4.00 | 6.96 | 6 | 3.44 | 1 | 10.70 | 7 | 5.30 | 3 | 4.94 | 2 | |
| r | 0.824 | 7 | 0.912 | 6.000 | 0.957 | 3 | 0.984 | 2 | 0.991 | 1 | 0.942 | 5 | 0.946 | 4 | |
| Mean of sub-ranks | 5.33 | 5.00* | 5.00* | 1.33 | 5.00* | 3.67 | 2.67 | ||||||||
| BK ranking | 5 | 4 | 4 | 1 | 4 | 3 | 2 | ||||||||
| NC + SOT | SD | 0.84 | 6 | 0.45 | 3.00 | 0.83 | 5 | 0.51 | 4 | 0.86 | 7 | 0.44 | 2 | 0.31 | 1 |
| CV | 4.12 | 7 | 2.13 | 4.00 | 3.61 | 6 | 1.90 | 3 | 3.21 | 5 | 1.80 | 2 | 1.30 | 1 | |
| r | 0.926 | 1 | 0.561 | 7.000 | 0.922 | 2 | 0.657 | 6 | 0.911 | 3 | 0.783 | 5 | 0.849 | 4 | |
| Mean of sub-ranks | 4.67* | 4.67* | 4.33* | 4.33* | 5.00 | 3.00 | 2.00 | ||||||||
| BK ranking | 4 | 4 | 3 | 3 | 5 | 2 | 1 | ||||||||
| PC + KG | SD | 1.03 | 5 | 0.27 | 2 | 1.45 | 7 | 0.57 | 4 | 1.04 | 6 | 0.32 | 3 | 0.27 | 1 |
| CV | 5.16 | 6 | 1.28 | 2 | 6.44 | 7 | 2.13 | 4 | 4.02 | 5 | 1.32 | 3 | 1.12 | 1 | |
| r | 0.991 | 1 | 0.819 | 6 | 0.982 | 2 | 0.921 | 4 | 0.969 | 3 | 0.885 | 5 | 0.743 | 7 | |
| Mean of sub-ranks | 4.00* | 3.33 | 5.33 | 4.00* | 4.67 | 3.67 | 3.00 | ||||||||
| BK ranking | 4 | 2 | 6 | 4 | 5 | 3 | 1 | ||||||||
SD—standard deviation, CV—coefficient of variation, r—coefficient of correlation.
* Same ranking for a given dataset