Table 6. Ranking of candidate genes according to expression stability values (SV) calculated by ΔCt method.
Sample set | Candidate reference gene | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
AfEF1a | AfGAPDH1 | AfGAPDH2 | AfTBP2 | AfTUA1 | AfUBC1 | AfUBC2 | ||||||||
SV | Rank | SV | Rank | SV | Rank | SV | Rank | SV | Rank | SV | Rank | SV | Rank | |
Total | 0.879 | 5 | 0.79 | 3 | 0.915 | 6 | 0.851 | 4 | 1.065 | 7 | 0.723 | 1 | 0.752 | 2 |
NPC | 0.854 | 5 | 0.79 | 4 | 0.857 | 6 | 0.715 | 2 | 0.92 | 7 | 0.698 | 1 | 0.734 | 3 |
NPC+SOT | 0.744 | 6 | 0.673 | 3 | 0.711 | 5 | 0.693 | 4 | 0.744 | 7 | 0.651 | 2 | 0.599 | 1 |
NC+S | 1.244 | 6 | 0.916 | 4 | 0.991 | 5 | 0.881 | 3 | 1.784 | 7 | 0.826 | 1 | 0.844 | 2 |
NC+SOT | 0.813 | 6 | 0.736 | 4 | 0.782 | 5 | 0.728 | 3 | 0.834 | 7 | 0.706 | 2 | 0.672 | 1 |
PC+KG | 0.705 | 4 | 0.693 | 3 | 0.935 | 7 | 0.690 | 2 | 0.794 | 6 | 0.682 | 1 | 0.755 | 5 |