Fig 7. Signalling-dependent activators are required for de-repression of a PRE in ph mutant clones.
(A-A”) The rpr(PRE)-GFP reporter is generally undetected in control eye discs with neutral clones. (B-B”) The reporter becomes activated in ph clones, but its expression is again reduced in clones expressing UAS-bskDN to block JNK signalling (C-C”). (D-D”) Similar abrogation of reporter expression in ph clones was observed when using a version with mutated binding sites for the canonical JNK transcription factor AP-1. DAPI staining (cyan) is shown in the left panels, while the central panels show the reporters and the distribution of RFP-marked clones is shown on the right panels. Scale bar represents 200 μm.