Extended Data Figure 6. Molecular profiling of rEC-HSPCs and rEC-HSCs.

a, Supervised principal component analysis of global gene expression data of rEC-HSPCs and rEC-HSCs and the indicated control cell types. Data for individual cells of given is indicated. Each shape represents independent replicate for the indicated cell types (embryonic day 11 (E11.0) aorta-gonad-mesonephros endothelium, E11.0 CD201⁻ pre-HSC type 1, E11.0 CD201⁺ pre-HSC type 1, E11.0 CD201⁺ pre-HSC type 2, E12.5 fetal liver HSCs (lin⁻Sca-1⁺Mac1^loCD201⁺), E14.5 fetal liver HSCs (lin⁻CD45⁺CD150⁺CD48⁻CD201⁻), adult bone marrow HSC (LKS-SLAM), ‘sc-’ refers to the single-cell RNA-seq data set adapted from ³¹; adult lung endothelial cells, in vitro rEC-LKS, in vivo rEC-LKS-SLAM, control adult in vivo LKS-SLAM cells). b. Supervised clustering of canonical endothelial genes expression profiles of freshly isolated lung endothelial cells (n = 3), embryonic stem cell (ES)-derived endothelial cell (n = 2), embryonic stem cells (n = 1); rEC-LKS-SLAM cells isolated from transplanted mice (n = 3), (n = 3, n = 4 from Kinkel et al ³², day 28 in vitro rEC-LKS (n = 3), data points obtained from clonal reprogramming are denoted by an asterisk. c, Supervised clustering of prototypical haematopoietic genes expression, demonstrates that haematopoietic genes are induced during Fgrs-mediated reprograming of endothelial cells into rEC-HSPCs and rEC-HSCs. d, Supervised clustering of prototypical pluripotency genes expression, demonstrates that pluripotency genes are not induced during Fgrs-mediated reprograming of endothelial cells into rEC-HSPCs and rEC-HSCs. e, Dendrogram showing unsupervised hierarchical clustering of global gene expression data of representative control cells (Ctl), and all rEC-HSCs, rEC-HSPCs, and their progenies. Dendrogram branches are colour-coded per cell types indicated in the legend.