Figure 2. Conditional Fgrs expression supports long-term primary and secondary HSPC engraftment.

a, Transplantation schema. b, Lineage contribution to Gr1⁺CD11b⁺ and Gr1⁻CD11b⁺ myeloid cells, B220⁺ B cells, CD3⁺CD4⁺ T cells, and CD3⁺CD8⁺ T cells at week 20 after primary transplant in the peripheral blood of WBM control transplant recipients (blue circles) or rEC-HSPC recipients (green circles), Boxplot and whiskers represent median, 25^th and 75th percentile, mean is represented by + sign (n = 4 independent conversion experiments run in technical triplicates for each condition); two-tailed unpaired t-test. c, Lineage contribution to Gr1⁺CD11b⁺ and Gr1⁻CD11b⁺ myeloid cells, B220⁺ B cells, CD3⁺CD4⁺ T cells, and CD3⁺CD8⁺ T cells at week 20 after secondary transplant in the peripheral blood of WBM control transplant (blue circles) or rEC-HSPC (green circles), Boxplot and whiskers represent median, 25^th and 75th percentile, mean is represented by + sign (n = 4 independent conversion experiment run in technical triplicates for each conditions); two-tailed unpaired t-test. d, Relative representation of LKS and LKS-SLAM cells at week 20 after primary transplant for WBM transplant recipients (blue circles) or rEC-HSPC recipients (green circles). e, Relative representation of LKS and LKS-SLAM cells at week 20 after secondary transplant for WBM transplant recipients (blue circles) or rEC-HSPC recipients (green circles). Boxplot and whiskers represent median, 25^th and 75th percentile, mean is represented by + sign (n = 4 independent conversion experiment run in technical triplicates for each conditions); two-tailed unpaired t-test