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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Nature. 2017 May 17;545(7655):439–445. doi: 10.1038/nature22326

Figure 4. Gene expression profiling of rEC-HSPCs and rEC-HSCs.

Figure 4

Principal Component Analysis (PCA) of the transcriptome-wide RNA-Seq expression profiles was performed. A 3D-PCA plot, representing ~85.0% of the total variation within the expression dataset, was generated to visualize the clustering of the reprogrammed hematopoietic sample groups in relation to their native counterparts. Three dimensional MDS plots (3D-MDS) were generated based on all pairwise distances between the global transcriptome-wide RNA-seq profiles of the samples shown here. Distances were defined as one minus the Pearson correlation between two profiles. Multidimensional scaling (MDS) was used to identify the set of points in 3D space, such that the distances between the points are equal to the true distances between samples.