Table 1.
Inflammatory cytokine profiling in serum of mice inoculated orally with virus
| Conc. In 100 μl mouse serum (the ELISA was run on a 10E-1 dilution)
| ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cytokine | IL1 α | IL1 β | IL2 | IL4 | IL6 | IL10 | IL12 | IL17A | IFN-γ | TNF-α | G-CSF | GM-CSF |
| − Control | 0·07 | 0·089 | 0·054 | 0·049 | 0·108 | 0·061 | 0·086 | 0·052 | 0·06 | 0·058 | 0·064 | 0·119 |
| 6 h Tris | −0·08 | −0·01 | −0·06 | 0·02 | −0·22 | 0·13 | −0·21 | 0·28 | 0·03 | 0·02 | 0·07 | −0·12 |
| 6 h SMV1 | −0·02 | 0 | −0·12 | −0·07 | −0·37 | 0·12 | −0·2 | 0·05 | 0·87 | −0·02 | 0·09 | 0·07 |
| 6 h M13KE | 0·06 | 0·6 | 0·06 | 0·05 | −0·24 | 0·14 | −0·06 | 0·31 | 0·05 | 0·02 | −0·04 | 0·12 |
| 12 h Tris | −0·01 | −0·2 | 0 | 0·04 | −0·29 | 0·13 | −0·19 | 0·12 | 0·16 | 0·18 | −0·02 | −0·04 |
| 12 h SMV1 | 0·05 | −0·04 | 0·13 | 0·12 | −0·2 | 0·2 | −0·14 | 0·26 | 0·13 | 0·2 | 0·11 | 0·07 |
| 12 h M13KE | 0·04 | 0 | 0·04 | 0·06 | −0·29 | 0·16 | −0·15 | 0·2 | −0·11 | 0·21 | 0·01 | 0·11 |
| + Control | 1·65 | 1·05 | 1·83 | 1·87 | 1·13 | 0·83 | 1·41 | 2·45 | >2·5* | 1·70 | 0·29 | 1·82 |
*
Absorbance values >2·5 are not within the linear range of the assay. Lower limit of detection; values less than two times the negative control should not be interpreted. Absorbance read at 450 nm.
The serum levels of 12 pro-inflammatory cytokines measured under identical conditions in experimental animals at time points 6 h and 12 h postoral administration. Numbers in bold are significantly higher than detection limit.